Making a dilution series

aim 

to make a dulution series to investigate concertration 

Equipment 

A potassium permanganate crystal siz large test tubes tweezers a plastic transfer pipette a test rack 10ml measuring cylinder 

method 

1.  place the six large test tubes in a test tube rack label the rack with number 1-6

2.using the measuring cylinder fill the tube 1 with 10 ml of water fill the remaining test tubes with 5ml of water 

3.using your tweezers add a single crystal of potassium permanganate to test tube 1

4.gently shake the test tube until the crystal has dissolved 

5.using the transfer pipette carefully remove exactly 5ml from the test tube 1 and pour it into test tube 2 

6.rinse the transfer pipette thoroughly to ensure that no purple solution remains 

7.gently shake test tube 2 and repeat the transfer progress transferring exactly 5 ml of solution from test tube 2 to test tube 3 

8. rinse the pipette again and repeat the transfer progress for test tube 4 5 and 6   



results

we failed because we put to much water in each test tube but it turned out all right after all.